, (76 cm) with BD FACS , Loader Option installed, Weight Cytometer 320 lb (146 kg) , Abstract As with many aspects of the validation and monitoring of flow cytometric methods, the method transfer processes and acceptance criteria described for other technologies are not fully appli. Operating instructions are contained in the BD, The BD FACSVerse flow cytometer is covered by one or more of the following US patents: 7,787,197; BD FACSVerse System User's Guide. W5: Massenspektrometrie (Theorieworkshop mit Anwendungsbeispielen) W5. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific). 6 Getting Started with BD FACSDiva Software Administrative Options User profiles can be exported and imported to assist with sharing profiles between workstations. 1 Joannem Research Forschungsgesellschaft mbH, HEALTH - Institut fr Biomedizin und Gesundheitswesen, Graz, sterreich. Immunogen corresponding to tissue, cells or virus. BD FACSCanto II. Keywords. It features many in. BD FACS Canto II, 3 laser (405nm, 488nm, 633nm) 10 parameter, technical specifications sheet, optical configuration Canto II, Search, Organize your work, Online Scheduling, How to get access to our file server, Internal Link, Resources for panel design, Fluorofinder, OMIPS, MACS Pick Up Point, Web Portal, Cytometry A, Journal, The optics of the BD FACSCanto II system consist of an excitation source with up to three lasers, a blue (488-nm, air-cooled, 20-mW solid state), a red (633-nm, 17-mW HeNe) and a violet (405-nm, 30-mW solid state). BD FACSCanto IIInstructions For UseIVDFor In Vitro Diagnostic Usebdbiosciences.comPart No. Built on more than 25 years of BD experience and leadership in ow cytometry and multicolor analysis, the BD FACSCanto II system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy, and high-quality results. IVD For In Vitro Diagnostic Use bdbiosciences.com Part No. Mass spectrometry is an analytical technique which separates molecules according to their mass: charge ratio and provides . 642239 Rev. Mouse monoclonal CD105 antibody [2H6F11] conjugated to PerCP. 1.4. Organization . Atlanta Pediatric Research | Emory + Children's + GT Pediatric Research . Page 1 of 5 Sponsored and reviewed by ICCS Quality and Standards Committee Title: Instrument Installation, Operational, and Performance Qualification for BD FACS Canto II Written by: Brahmananda R. Chitteti and Virginia Litwin Date: 01 Oct 2018 Introduction BD FACSCanto II is a flow cytometer intended for the qualitative and quantitative measurement of Cited in 1 publication(s). Validated in Flow Cyt and tested in Human. SRC | A | W56HZV-16-R-L147: Sources Sought | Live Fire Test and Evaluation - Market Survey | Department of the Army, Army Contracting Command, ACC - WRN (W56HZV) (DTA) SRC | A | NRL-16-MV03: Sources Sought | Research and Development Services for Vacuum Electronics Theoretical Models and . BD FACSCanto II Instructions For Use, IVD, For In Vitro Diagnostic Use, bdbiosciences.com Part No. Purpose . Massenspektrometrie (Theorieworkshop mit Anwendungsbeispielen) A. Mautner 1. Read Bd FACS Canto II Operator's Manual - Marshall Scientific by Marshall Scientific on Issuu and browse thousands of other publications on our pla. In our study, we performed an inter-laboratory comparison of EV isolation based on a differential ultracentrifugation protocol carried out in 4 laboratories in 2 independent rounds of isolation . 1.1. Name: Baylor Institute for Immunology Research Flow cytometry is a robust technology that can be used for several applications in oncology, hematology and immunology. The BD FACSCanto II System features a fixed-alignment flow cell in the fluidics system that minimizes startup time and improves reproducibility. 1.4.1. Laser Detection Filter Colours 525/50 FITC, BB5815, VioBright FITC, Alexa488 576/26 PE, DS Red 695/40 PerP, PerP-y., PE-y. (P.), PerP-eFluor , PE-Alexa Fluor , PE-Alexa Fluor , PE-Alexa , (61 x 91 x 64 cm) , Fluidics cart 24 x 31 x 25 in. The BD FACSCanto II Flow Cytometry system delivers flow cytometry data on an efficient user friendly platform. BD FACSCanto II System, Innovation with Impact, Specifications, Installation Requirements , Size (D x W x H) Cytometer 24 x 36 x 25 in. A Waters 1525 LC pump is used to deliver the mobile phase to the source, and samples are selected and injected using a Waters 2777 autosampler.",Electrospray Mass Spectrometry,,Dr Lisa Haigh,,,l.haigh@imperial.ac.uk,Mr Timothy Jefferson,,,t.jefferson@imperial.ac.uk,,,FoNS . more distance between the peaks) Bad resolution (CV) of the population may cause you to miss information because it is hidden by the overlapping populations. The U266 cell line was purchased from the American Type Culture Collection (Manassas, VA) and cultured as previously described [] using RPMI media (Gibco/Life Technologies; Grand Island, NY) supplemented with 10% fetal serum, penicillin/streptomycin, and IL-6 (R&D Systems; Minneapolis, MN).Bortezomib (Millennium Pharmaceuticals; The Takeda Oncology Company; Cambridge . FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences). Low Prices 100% Guarantee AJune 2007BD Biosciences2350 Qume DriveSan Jose, CA 95131-1807USATel (877) 232-8995Fax (800) 325-9637facservice@bd.comECREPBENEX LimitedBay K 1 a/dShannon Industrial EstateShannon, County ClareIrelandTel (353) 61-472920Fax (353) 61-472546CanadaToll Free (888) 259-0187Tel (905) 542-8028Fax . Its flexible modular design offers automation and up to eight-color capacity for rapid and easy production of high-quality data in clinical and research laboratories. 1.2. Instructions For Use. Their low size range (0.1 - 1m) creates a difficult challenge for detection, sizing and counting by flow cytometry1.A standardized protocol for PMP counts in the limited size range of 0.5 to 1m2 had been developed on Beckman-Coulter (BC) FC500 cytometers (FCMrs).Recent studies3 showed that this strategy, optimized on BC FCMrs measuring Forward. Title: Instrument Installation, Operational, and Performance Qualification for BD FACS Canto II with general cytometer functions as described in your. Introduction. 642239 Rev. This manual contains reference information about the BD FACSCanto II flow cytometer and the BD FACS Loader. Sponsored and reviewed by ICCS Quality and Standards Committee. A June 2007, BD Biosciences 2350 Qume Drive San Jose, CA 95131-1807 USA Tel (877). , (61 x 79 x 64 cm) , The cytometer depth increases to 30 in. This slide represents the difference between the term resolution, sensitivity, and dynamic range very well. A good resolution (better CV) versus the sensitivity (i.e. Tested Reactivity: Human. The instrumentation for clinical applications has evolved from 2 color to 4 color and now 10 and 12 color. To increase sensitivity and resolution for each color in a multicolor assay, a patented optical design maximizes signal detection. FBODaily.com | FedBizOpps Issue of 27-Jul-16 | FBO#5360. Buy rabbit monoclonal (T22-A) antibody to Bax (A8302). BD FACSCanto II Flow CytometerTechnical Specifications, Optics, Lasers, Air-cooled: 488-nm solid state, 20-mW laser output 633-nm HeNe, 17-mW laser output, Laser Configuration, Spatially separated beams with 9 x 65- m elliptical spots, Optical Alignment Procedure, Fixed, no operator alignment required, Flow Cell, The BD FACSCanto II System's high sensitivity delivers accurate results, Developing a new 10 color assay or converting an existing 5 color assay into 10 color is appealing on many fronts . A June 2007. 1. INSERT INTO `equipments` (`name`, `brand`, `serial`, `vendor`, `inv_ref`, `comments`, `location`, `manager`, `date_aquisition`, `cat`, `specification` ) VALUES . Conditioned medium of colorectal cancer cells was prepared and pooled by 1 person and distributed to each of the participating laboratories for isolation according to a pre-defined protocol. Other added functions of the BD FACSCalibur include optical coupling, background rejection, clear signal detection, and red-diode (635-nm) laser signal rejection, among others. The BD FACSCanto II can be congured with two or three lasers to detect up to eight colors. 1.3. Administrators can control user access to certain instrument settings Methods. Cell lines and culture. BD Biosciences EC REP BENEX Limited Canada Japan 5ppm can be carried out on this instrument, and LC/MS is also available. Final instrument fc500. Experiment Variables. Experiment Overview . Technical Specifications, 642239 Rev. Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the commonest erythrocytic enzymopathy with approximately 400 million people affected worldwide [].Being an X-linked recessive trait, the majority of deficient individuals are hemizygous males. AJune 2007BD Biosciences2350 Qume DriveSan Jose, CA 95131-1807USATel (877) 232-8995Fax (800) 325-9637MexicoToll Free 01-800-236-2543JapanNippon Becton Dickinson CanadaToll Free (888) 259-0187Fax (888) 229-9918Tel (905) 542-8028canada@bd.comAsia PacificFax (65) 6-860-1590Tel (65) 6-861-0633Brazil Tel (55) 11-5185-9995Fax (55) 11-5185-9895BENEX Li. Validated Applications: FC. Read "Congress of Clinical Chemistry and Laboratory Medicine, Clinical Chemistry and Laboratory Medicine (CCLM)" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. The BD FACSCalibur system is fully modular, thus allowing easy upgrade to satisfy specific application needs. For rapid and easy production of high-quality data in clinical and research laboratories increases to 30. Analytical technique which separates molecules according to their mass: charge ratio and provides 2350 Qume Drive Jose. 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